CIRCUM SPOROZOITE PROTEIN DETECTION IN MOSQUITO SPECIES MALARIA VECTOR Anopheles vagus SUSPECTED IN KOKAP SUBDISTRICT, KULON PROGO REGENCY WITH ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

R. A. Wigati, Mardiana ., Mujiyono ., Siti Alfiah

Abstract


Anopheles species as malaria vector, as if tested in the salivary gland containing sporozoites existence which could be checked in the mosquito salivary gland and Enzyme-Linked Immunosorbent Assay (ELISA). This study aimed to investigate the circum sporozoite protein in the mosquito of Anopheles vagus with Enzyme-Linked  Immunosorbent Assay (ELISA). The study was conducted in malaria endemic area namely Hargorejo, Kalirejo, Hargowilis, and Hargotirto villages, Kokap subdistrict, Kulon Progo Regency in June-October 2005. The study design was cross-sectional study. ELISA performed on the An.vagus which is in ovaries shown parous. An.vagus parous body parts for the ELISA are the head-thorax, where it is possible to contain the sporozoites of Plasmodium falciparum or Plasmodium vivax. The results of ELISA to An.vagus in four research areas, showed that 41 samples of An.vagus mosquitoes in Hargorejo village, three positive (7,32%) circum sporozoites protein of Plasmodium falciparum, five samples of An.vagus mosquito in Kalirejo village, there is one positive (20%) circum sporozoites protein of P.falciparum. 16 samples of An.vagus mosquito in Hargowilis village, found one positive (6,25%) circum sporozoite protein of P.falciparum, one sample of An.vagus mosquito in Hargotirto village was not found circum sporozoite protein of P.falciparum (0%) and circum sporozoite protein of P.vivax (0%). The number of samples from four villages are 63 samples of mosquitoes, found five positive circum sporozoites protein of P.falciparum (7,94%) and not found circum sporozoite protein of P.vivax (0%). The results of ELISA showed more specimens found positive in mosquitoes containing circum sporozoite protein of P.falciparum in the animal cage, not in human, there were because of choice possibility is not selective. At a wavelength of 405 nm in the ELISA  results were absorbent for An.vagus mosquitoes tested positive, ranging from 0,257 to 0,632. Value of absorbent positive control was 0,449 and negative control absorbent value were 0,208; 0,228; 0,202; 0,204; 0,207; 0,223; 0,206. The value of cut-off point is taken from the highest absorbent value of negative control is 0,228.

Keywords: Detection, Circum Sporozoite Protein, An.vagus, Kokap subdistrict, Enzyme-Linked Immunosorbent Assay (ELISA)

References



Full Text: Full text in Indonesian

Refbacks

  • There are currently no refbacks.